Program Overview

Appropriate Technology Practice Course includes

 

Techniques 

  1. Fungal collection and single spore/hyphae isolation
  2. Microscopy (stereo and upright)
  3. PCR
  4. Microorganism culturing and handling
  5. Bioreactor and fermenter
  6. Analytical HPLC
  7. Mass spectrometry
  8. Cell culture
  9. Spectrophotometer
  10. Flow cytometer
  11. Cell cytoxicity assay (MTT)
  12. Transwell/time-lapse microscopy for cell migration
  13. Zebrafish technology
  14. Animal caring and oral gavage
  15. Paraffin embedding and microtome
  16. H&E staining 

Participating laboratories

Dr. Tang-Long Shen (Cancer Cell Biology)
Dr. Shih-Torng Ding (Nutritional Molecular Biology)
Dr. Shean-Shong Tzean (Mycology)
Dr. Tsai-Kun Li (Chromatin Biology and DNA Topoisomerases)
Dr. Hsinyu Lee (Endocrinology)
Mucho Ltd. Inc., Taiwan (Industry)

Program

  1. Field trip --- SST/TLS/Mucho
  2. Laboratory identification (including documentation, photography, isolation, culture, and genotyping -- SST /TLS
  3. Cultivation and fermentation --- SST /TLS
  4. Chemical analyses --- TLS
  5. in vitro bioassays (MTT, migration, whitening, ROS, lipolysis, anti-virus) --- TLS/TKL/HL
  6. Toxicological test --- TLS/SSD
  7. Industrialization --- Mucho
  8. Report written
  9. Result discussion

Credits 


Applied Translational Microbiology (3 credits)

Update: 2016 / 10 / 28